Lamin B2 (LMNB2) antibody

Antigen: Lamin B2 (LMNB2)

Synonyms: LMN2, LAMB2, MGC2721, lamin, RGD1563803, lmnb2-a, LMNB2, lmn2, lamb2, Lamin-L(II), LOC100136040

Clonality: Monoclonal (LN43)

Host: Mouse

Reactivity: Hamster

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN360851

Additional Information

Characteristics: Synonyms: Lamin-B2, LMNB2, LMNB-2, LMN2, LMNB, Nuclear Envelope Marker

Alternative name: Lamin-B2 (LMNB2)

Swiss-Prot: Q03252

Immunogen: Detergent insoluble fraction of potoroo cell line PtK1.

Cross-Reactivity: Hamster, Human, Mouse (Murine), Xenopus laevis

Isotype: IgG1  (Matching secondary antibodies)

Clone: LN43

Description: An important part of the cell nucleus is formed by nuclear lamina. Nuclear lamins form anetwork of filaments at the nucleoplasmic site of the nuclear membrane. Two mainsubtypes of nuclear lamins can be distinguished, i. e. A-type lamins and B-type lamins. TheA-type lamins comprise a set of three proteins arising from the same gene by alternativesplicing, i. e. lamin A, lamin C and lamin Adel10, while the B-type lamins include twoproteins arising from two distinct genes, i. e. lamin B1 and lamin B2. The nuclear laminscomprise a unique subclass of the intermediate filament protein family. They share amolecular domain organisation with the other intermediate filament proteins in that theyare fibrous molecules that have an aminoterminal globular head, a central rod of a-helicesand a carboxyterminal globular domain. Many biochemical and molecular features oflamins have been studied, but their functions remain still largely undetermined. One of thefunctions ascribed to the lamina is the maintenance of the structural integrity of thenucleus. Besides interactions with the nuclear membrane and other intermediatefilaments, lamins interact with the nuclear chromatin. Eukaryotic chromatin is organisedinto loops, which are attached to the nuclear matrix. This organisation is thought tocontribute to compaction of the chromatin and regulation of gene expression. Lamins, aspart of the nuclear matrix, may be involved in these processes since chromatin bindingsites have been detected in both A- and B-type lamins.

Specificity: Reacts with an epitope located in the C-terminal part of Lamin B2. Species: Human, Mouse, Hamster and Xenopus. Others not tested.

Application Details

Application Notes: Suitable for Immunocytochemistry on Frozen Sections, Immunoblotting and FlowCytometry. Recommended Dilutions: 1/100-1/200 for Flow cytometry and for Immunohistochemistry with avidin-biotinylatedhorseradish peroxidase complex (ABC) as detection reagent.1/100-1/1000 for Immunoblotting applications. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS with 0.09% Sodium Azide as preservative.

Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Research Area: Cytoskeleton

Restrictions: For Research Use only