SERPINA1 antibody

Catalog No. ABIN360977

The Rabbit Polyclonal anti-SERPINA1 antibody is suitable to detect SERPINA1 in samples from Human. It has been validated for WB, IHC (p) and EIA.

Quick Overview for SERPINA1 antibody (ABIN360977)

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SERPINA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SERPINA1 Antibody

Purification: Immunoaffinity Chromatography using antigen resins attached to a solid phase.

Immunogen: Human Alpha-1-anti-Trypsin

Application Details

Application Notes: Suitable for most immunological methods. This product has been assayed against 1.0 μg of Human Alpha-1-anti-Trypsin in a standardsandwich ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG [H&L]MX and TMB as a substrate for 30 minutes at room temperature. A working dilution of1: 10,000 to 1: 50,000 is suggested for this product. Recommended Dilutions: ELISA : 1/10,000-1/600,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/500-1/2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.41 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Target Details for SERPINA1

Target: SERPINA1 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 1 (SERPINA1))

Alternative Name: alpha-1-Antitrypsin

Background: Alpha 1 Antitrypsin is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase.Synonyms: AAT, Alpha-1 protease inhibitor, Alpha-1-antiproteinase, SERPINA1

Gene ID: 9606

UniProt: P01009