Patatin-Like Phospholipase Domain Containing 3 (PNPLA3) antibody

Antigen: Patatin-Like Phospholipase Domain Containing 3 (PNPLA3)

Synonyms: ADPN, C22orf20, iPLA(2)epsilon, Adpn, PNPLA3, LOC100231669

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN361184

Additional Information

Alternative name: PNPLA3 / Adiponutrin

Gene ID: 80339, 116939 (mouse), 362972 (rat)

Swiss-Prot: NP_473429.2

Immunogen: Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine)

Format: Liquid

Description: Principal Names: adiponutrin, Adpn, patatin-like phospholipase domain containing 3, Pnpla3. Official Symbol: PNPLA3

Specificity: Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

Molecular Weight: 42.6kDa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 45kDa band observed in Mouse Heart lysates (calculated MW of 42.6kDa according to Human NP_473429.2 and to Mouse NP_473429.2). Recommended concentration: 0.05-0.2µg/ml. IHC: In paraffin embedded Human Skin shows membranous staining of basak kayer cells. Recommended concentration, 4-6µg/ml.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only