E2F Transcription Factor 7 (E2F7) antibody

Antigen: E2F Transcription Factor 7 (E2F7)

Synonyms: FLJ12981, D10Ertd739e, A630014C11Rik, E2F7, fc43h03, wu:fc43h03, si:dkey-217k21.2, MGC154335

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN361188

Additional Information

Alternative name: E2F7

Gene ID: 144455

Immunogen: Peptide with sequence C-DLSKQKKFTPERNP, from the internal region of the protein sequence according to NP_976328.2

Description: All names and symbols: E2F7, E2F transcription factor 7, FLJ12981. Official Symbol: E2F7. RefSeq number(s): NP_976328.2.

Specificity: Expected from sequence similarity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 70kDa band in Human Skin lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 99.9kDa according to NP_976328.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only