Phosphoserine Phosphatase (PSPH) antibody

Antigen: Phosphoserine Phosphatase (PSPH)

Synonyms: PSP, PSPase, AI480570, MGC109524, PSPH, psph, MGC78828, MGC137208, MGC112414, DKFZp469L1337, DKFZP468A2313

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN361197

Additional Information

Alternative name: PSPH

Gene ID: 5723, 100678 (mouse)

Immunogen: Peptide with sequence C-RQQVKDNAKWYITD, from the C-Terminus of the protein sequence according to NP_004568.2

Description: All names and symbols: PSPH, phosphoserine phosphatase, PSP, L-3-phosphoserine phosphatase, O-phosphoserine phosphohydrolase, OTTHUMP00000025059, PSPase. Official Symbol: PSPH. RefSeq number(s): NP_004568.2.

Specificity: Expected from sequence similarity: Human, Mouse, Rat, C-Terminus

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 26kDa band observed in Human Brain (Hippocampus) and in Mouse brain lysates (calculated MW of 25.0kDa according to Human NP_004568.2 and 25.1kDa according to Mouse NP_598661.1). Recommended concentration: 0.05-1µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

EB09299 (0.05µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.