4-Hydroxy-2-Hexenal (4-HHE) antibody

Catalog No. ABIN361275

Cited in 2+ publications. The Mouse Monoclonal anti-4-Hydroxy-2-Hexenal (4-HHE) antibody (Clone HHE53) (ABIN361275) specifically detects 4-Hydroxy-2-Hexenal (4-HHE) in IHC.

Quick Overview for 4-Hydroxy-2-Hexenal (4-HHE) antibody (ABIN361275)

Target: 4-Hydroxy-2-Hexenal (4-HHE)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Clone: HHE53

Product Details

Specificity: Specific for HHE-modified protein (especially HHE-His Michael adduct)

Immunogen: HHE-modified keyhole-lympet hemocyanine

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry: It is recommended that the antibody be tried at 0.5-1.0 micro g/mL on paraformaldehyde fixed tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Frozen in 10 mM PBS containing 0.1 % NaN3 and 0.1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Tanito, Elliott, Kotake, Anderson: "Protein modifications by 4-hydroxynonenal and 4-hydroxyhexenal in light-exposed rat retina." in: Investigative ophthalmology & visual science, Vol. 46, Issue 10, pp. 3859-68, (2005) (PubMed).

Yamada, Funada, Shibata, Kobayashi, Kawai, Tatsuda, Furuhata, Uchida: "Protein-bound 4-hydroxy-2-hexenal as a marker of oxidized n-3 polyunsaturated fatty acids." in: Journal of lipid research, Vol. 45, Issue 4, pp. 626-34, (2004) (PubMed).

Target Details

Target: 4-Hydroxy-2-Hexenal (4-HHE)

Target Type: Chemical

Background: 4-hydroxy-2-alkenal is one of the major lipid peroxidation products, and shows many biological effects such as high toxicity to cells. Among them, 4-hydroxy-2-hexenal (HHE) is an aldehyde formed during peroxidation of n-3 fatty acids such as docosahexaenoic acid. HHE is highly reactive aldehyde and reacts with histidine residue of protein to form Michael-addition type adducts. This antibody is specific for HHE-histidine Michael adduct (HHE-His) and enable to detect HHE-His in the tissue samples.