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Antibody
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anti-Tubulin Beta 3 TUBB3
Tubulin, Beta, 3 (TUBB3) antibody
| Antigen |
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| Synonyms |
TUBB3, TUBB4, MGC134115, MC1R, beta-4, tbb3, M(beta)3, M(beta)6, 3200002H15Rik, tubb3, MGC76202, TUBB1, tubB3, tubulin |
| Clonality |
Monoclonal (AA10) |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB) (17), Immunofluorescence (IF) (13), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Flow Cytometry (FACS) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunocytochemistry (ICC) (4), ELISA (3), Immunohistochemistry (IHC) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Radioimmunoassay (RIA) (1)
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| Catalog no. |
ABIN361368 |
| Quantity |
100 µl |
| Price |
345.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business days |
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Alternative name
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Beta III Tubulin
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Isotype
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IgG (Matching secondary antibodies)
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Clone
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AA10
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Description
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Tubulin is the major constituent of microtubules, existing as a heterodimer of the alpha and beta subunits. The beta III isoform of tubulin is found almost exclusively in neuronal processes of adult tissues and is therefore an excellent marker for neurons. Neuron specific, posttranslational modifications within the C-terminal domain of beta III tubulin have been shown to be developmentally regulated suggesting that they may serve to modulate the interaction of tubulin with microtubule associated proteins (Lee et al., 1990). Additionally, beta III tubulin has been found to be highly expressed in cancer cells such as small cell lung cancer, large cell neuroendocrine carcinoma and adenocarcinomas and is correlated with an increasing histological degree of malignancy (Katsetos et al., 2003) Anti-Beta III Tubulin Western blot of rat cortex lysate showing specific immunolabeling of the ~55k beta III tubulin protein.
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Specificity
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Specific for the ~55kDa beta III tubulin protein. This clone is similar to the monoclonal antibody Tuj1. It is expected that this antibody will have wide species cross reactivity.
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Application Notes
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Recommended Dilution: WB: 1:10,000 IHC: 1:1,000 Quality Control: Western blots performed on each lot.
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Purification
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Protein G purified culture supernatant.
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Storage
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Store at -20 °C, stable for 1 year
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Restrictions
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For Research Use only
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Alternatives for antigen "Tubulin, Beta, 3 (TUBB3)", type "Antibodies"
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Hosts
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Mouse (18), Rabbit (8), Chicken (1)
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Reactivities
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Human (20), Rat (Rattus) (11), Mouse (Murine) (10), All Species (3), Various Species (3), Chicken (2), Cow (Bovine) (1), Hamster (1), Monkey (1), Pig (Porcine) (1)
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Applications
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Western Blotting (WB) (17), Immunofluorescence (IF) (13), Enzyme Immunoassay (EIA) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Flow Cytometry (FACS) (5), Immunohistochemistry (Frozen Sections) (IHC (fro)) (5), Immunocytochemistry (ICC) (4), ELISA (3), Immunohistochemistry (IHC) (2), Immunoprecipitation (IP) (2), ELISA (Detection) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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FITC (2), Alexa Fluor 488 (1), DY547 (1), Dyomics 547 (1)
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Epitopes
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AA 26-39 (1), pSer172 (1)
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