Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody

Antigen: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)

Synonyms: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Clonality: Monoclonal

Host: Mouse

Reactivity: Cow (Bovine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN361377

Additional Information

Alternative name: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)

Isotype: IgM  (Matching secondary antibodies)

Description: Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. Because GAPDH is a protein expressed in large amounts and which is required at all times for important house keeping functions, levels of GAPDH mRNA are often measured and used as standards in studies of mRNA expression. Increasingly, scientists are making use of specific antibodies to GAPDH in comparable studies of levels of protein expression. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription (1). GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease, and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease (2,3). Associations with actin and tubulin have also been reported. The protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic (4). Purification: Unpurified, concentrated culture supernatant.

Specificity: Specific for the ~38k Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.

Synonyms: G3PD, GAPD, MGC88685, Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, GA3PDH, GADPH, GAP, GAPDH, GAPDH II, GAPDH2, Gapdh-2, Gapdh13F, DmelCG8893, CG8893, BEST:GH12586, GAPDH I, GAPDH-1, Gapdh43E, gh12586, DmelCG12055, CG12055, MGC127711, gapd, cb609, wu:fb33a10, KNC-NDS6, g3pd, MGC89215, G3PDH, DKFZp468E2417, MGC53986, GAPC-2, GAPC2, GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE C-2, glyceraldehyde-3-phosphate dehydrogenase C2, T6J4.17, T6J4_17

Application Details

Application Notes: Recommended Dilution: WB: 1:1,000 IF: 1:100 Quality Control: Western blots performed on each lot.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blot of rat hippocampal lysate showing the immunolabeling of ~38k GAPDH protein.

Human neuroblastoma SH-SY5Y cells stained with mouse anti-GAPDH (green), chicken antibody to neurofilament NF-H (catalog # 1451-NFH) (red) and DNA (blue).