Glycine Receptor, alpha 1 (GLRA1) antibody

Antigen: Glycine Receptor, alpha 1 (GLRA1)

Synonyms: STHE, MGC138878, MGC138879, ot, spd, nmf11, spasmodic, oscillator, B230397M16Rik, GLYRA1, [a]Z1, GLRA1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN361432

Additional Information

Alternative name: Glycine Receptor

Format: Affinity-purified

Description: Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001, Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002). Anti-Glycine Receptor Western blot of rat spinal cord showing specific immunolabeling of the ~48k (1- and (2-subunits of the glycine receptor. The labeling was absent from a rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.

Specificity: Specific for the ~48k a1- and a2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other glycine receptor subunits.

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Storage: Store at -20 °C, stable for 1 year

Research Area: Receptors

Restrictions: For Research Use only

Images

Western blots of rat spinal cord showing specific immunolabeling of the ~48k (1- and (2-subunits of the glycine receptor. The labeling was absent from a rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.