Dynamin (pSer778) antibody

Antigen: Dynamin

Synonyms: DNM, Dnm, AI838169, KIAA4093, mKIAA4093, anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, CG18102, dDyn, Ddyn, Ddyn3, Ddyn4, DmelCG18102, dyn, Dyn, DYN, l(1)shi, l(1)VII, Shi, dnm1, MGC69248, DNM1, MGC142803, Dnm1

Epitope: pSer778

Clonality: Polyclonal

Host: Sheep

Reactivity: Rat (Rattus)

Application: Western Blotting (WB)

Catalog no.: ABIN361465

Additional Information

Alternative name: Phospho-Ser778 Dynamin

Format: Affinity-purified

Description: Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001, Graham et al., 2002, Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates dynamin at Ser774 and Ser778 that are the phosphorylation sites on dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on dynamin is thought to play a key role in synaptic vesicle trafficking. Anti-Phospho Ser778 Dynamin Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment with (-Ptase.

Specificity: Specific for the ~95k dynamin protein phosphorylated at Ser778. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by (-phosphatase treatment.

Synonyms: DNM, Dnm, AI838169, KIAA4093, mKIAA4093, anon-WO0153538.12, anon-WO0153538.13, anon-WO0153538.14, CG18102, dDyn, Ddyn, Ddyn3, Ddyn4, DmelCG18102, dyn, Dyn, DYN, l(1)shi, l(1)VII, Shi, dnm1, MGC69248, DNM1, MGC142803, Dnm1

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Purification: Prepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.

Storage: Store at -20 °C, stable for 1 year

Restrictions: For Research Use only

Images

Western blots of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 dynamin. The immunolabeling is completely eliminated by treatment with (-Ptase.