Heat Shock Protein 90 (HSP90) antibody

Antigen: Heat Shock Protein 90 (HSP90)

Synonyms: swo1, hsp90, HSP90, htpG, SCBAC25F8.08, 23.m06066, 17.m07646, HSP90-1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Plasmodium falciparum

Catalog no.: ABIN361682

Additional Information

Alternative name: Hsp90

Immunogen: Recombinant full length PfHsp90

Format: Protein A purified

Description: HSP90 is an abundantly and ubiquitously expressed heat shock protein. It is understood to exist in two principal forms a and

Specificity: This protein A Affinity purified antibody detects an 86kDa protein, corresponding to P. falciparum Hsp90 on SDS PAGE immunoblot, in samples from P. falciparum origin. Species cross-reactivity: It is specific to P. falciparum and does not cross-react to Hsp90 from human, yeast and dictyostelium.

Application Details

Application Notes: 1:2000 dilution for western blot and a 1:50 dilution for IF.

Concentration: 1.56mg/ml

Buffer: PBS pH 7.4, 50% glycerol, 0.09% azide. Protein A affinity purified.

Storage: -20°C, 1 year+, shipped on cold packs or ambient

Research Area: Cancer, Heat Shock Proteins

Restrictions: For Research Use only

Images

PfHsp90, malarial parasite lysate

Publications

Publications: Minami, Kawasaki, Miyata et al.: "Analysis of native forms and isoform compositions of the mouse 90-kDa heat shock protein, HSP90." in: The Journal of biological chemistry, Vol. 266, Issue 16, pp. 10099-103, 1991 (PubMed).

Whitesell, Mimnaugh, De Costa et al.: "Inhibition of heat shock protein HSP90-pp60v-src heteroprotein complex formation by benzoquinone ansamycins: essential role for stress proteins in oncogenic transformation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 18, pp. 8324-8, 1994 (PubMed).

Pratt, Toft: "Steroid receptor interactions with heat shock protein and immunophilin chaperones." in: Endocrine reviews, Vol. 18, Issue 3, pp. 306-60, 1997 (PubMed).

Nemoto, Sato, Iwanari et al.: "Domain structures and immunogenic regions of the 90-kDa heat-shock protein (HSP90). Probing with a library of anti-HSP90 monoclonal antibodies and limited proteolysis." in: The Journal of biological chemistry, Vol. 272, Issue 42, pp. 26179-87, 1997 (PubMed).

Pratt: "The hsp90-based chaperone system: involvement in signal transduction from a variety of hormone and growth factor receptors." in: Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), Vol. 217, Issue 4, pp. 420-34, 1998 (PubMed).

Pearl, Prodromou: "Structure, function, and mechanism of the Hsp90 molecular chaperone." in: Advances in protein chemistry, Vol. 59, pp. 157-86, 2002 (PubMed).

Neckers: "Hsp90 inhibitors as novel cancer chemotherapeutic agents." in: Trends in molecular medicine, Vol. 8, Issue 4 Suppl, pp. S55-61, 2002 (PubMed).

Pratt, Toft: "Regulation of signaling protein function and trafficking by the hsp90/hsp70-based chaperone machinery." in: Experimental biology and medicine (Maywood, N.J.), Vol. 228, Issue 2, pp. 111-33, 2003 (PubMed).

Banumathy, Singh, Pavithra et al.: "Heat shock protein 90 function is essential for Plasmodium falciparum growth in human erythrocytes." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18336-45, 2003 (PubMed).

Arlander, Eapen, Vroman et al.: "Hsp90 inhibition depletes Chk1 and sensitizes tumor cells to replication stress." in: The Journal of biological chemistry, Vol. 278, Issue 52, pp. 52572-7, 2003 (PubMed).

Pavithra, Banumathy, Joy et al.: "Recurrent fever promotes Plasmodium falciparum development in human erythrocytes." in: The Journal of biological chemistry, Vol. 279, Issue 45, pp. 46692-9, 2004 (PubMed).