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Details for Product No. ABIN361689

Methylated Lysine (meLys) antibody (HRP)

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Antigen
Epitope
»Alternatives meLys
Reactivity
»Alternatives All Species
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
Application
»Alternatives Western Blotting (WB), ELISA
Pubmed 6 references available
Catalog no. ABIN361689
Quantity 400 µL
Price
582.25 $   Plus shipping costs $45.00
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Availability Will be delivered in 3 to 4 Business Days
Immunogen Methylated KLH
Isotype IgG
Specificity Detects proteins containing methylated lysine residues in SDS PAGE immunoblots.
Background Synonyms:
dimethylysine, methyl lysine, N epsilon dimethyl lysine, trimethyl lysine
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves methylation. Lysine can be methylated once, twice or three times by lysine methyltransferases. The transfer of methyl groups from S-adenosyl methionine to histones is catalyzed by enzymes known as histone methyltransferases. Histones which are methylated on certain residues can act epigenetically to repress or activate gene expression.The transcriptional repressor SUV39H1 can encode novel enzymes which selectively methylate histone H3 at lysine 9. SUV39H1 places a methyl marker on histone H3, which is then recognized by HP1 through its chromo domain. This model may also explain the stable inheritance of theheterochromatic state. Some studies have also speculated a stimulatory role for transcription bymethylated histone lyside 4 due to its presence at active transcription sites.
Research Area Metabolism, Cell Signaling
Application Notes Recommended Dilution: 1:2000-1:5000 dilution WB
Restrictions For Research Use only
Concentration 250 µg/ mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Storage -20 °C
Background publications Melcher, Schmid, Aagaard et al.: "Structure-function analysis of SUV39H1 reveals a dominant role in heterochromatin organization, chromosome segregation, and mitotic progression." in: Molecular and cellular biology, Vol. 20, Issue 10, pp. 3728-41, 2000 (PubMed).

Bannister, Zegerman, Partridge et al.: "Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain." in: Nature, Vol. 410, Issue 6824, pp. 120-4, 2001 (PubMed).

Zegerman, Canas, Pappin et al.: "Histone H3 lysine 4 methylation disrupts binding of nucleosome remodeling and deacetylase (NuRD) repressor complex." in: The Journal of biological chemistry, Vol. 277, Issue 14, pp. 11621-4, 2002 (PubMed).

Ng, Xu, Zhang et al.: "Ubiquitination of histone H2B by Rad6 is required for efficient Dot1-mediated methylation of histone H3 lysine 79." in: The Journal of biological chemistry, Vol. 277, Issue 38, pp. 34655-7, 2002 (PubMed).

Im, Park, Feng et al.: "Dynamic regulation of histone H3 methylated at lysine 79 within a tissue-specific chromatin domain." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 18346-52, 2003 (PubMed).

Yang: "Multisite protein modification and intramolecular signaling." in: Oncogene, Vol. 24, Issue 10, pp. 1653-62, 2005 (PubMed).

Alternatives for antigen "Methylated Lysine", type "Antibodies"
Hosts (17)
Reactivities (14), (1), (1), (1), (1), (1)
Applications (17), (17), (10), (2), (2), (1), (1)
Conjugates (5), (4)
Epitopes (3), (2)
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