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Lysine (lys) (acetylated) antibody (Biotin)

lys WB, ICC, IF, IP, ELISA Host: Rabbit Polyclonal Biotin
Catalog No. ABIN361691
  • Target
    Lysine (lys)
    Binding Specificity
    • 22
    • 11
    acetylated
    Reactivity
    Please inquire
    Host
    • 21
    • 12
    Rabbit
    Clonality
    • 21
    • 12
    Polyclonal
    Conjugate
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    Biotin
    Application
    • 33
    • 32
    • 32
    • 32
    • 32
    • 11
    • 1
    Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA
    Specificity
    Detects proteins containing acetylated lysine residues. No reaction to non-acetylated proteins.
    Purification
    Protein A Purified
    Immunogen
    Acetylated KLH Conjugated
  • Application Notes
    • WB (1:250)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    A 1/250 dilution of SPC-156 was sufficient to detect the acetylated histone from TSA treated mouse spleen cell in western blot analysis.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    Lysine (lys)
    Alternative Name
    Lysine
    Synonyms
    lysine antibody, lys antibody
    Target Type
    Amino Acid
    Background
    Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anti-cancer drugs currently in development (5).
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