EC SOD antibody

Antigen: EC SOD

Clonality: Monoclonal (4GG11G6)

Host: Mouse

Reactivity: Human, Rat (Rattus), Mouse (Murine)

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN361742

Additional Information

Characteristics: Detects EC SOD at ~35kDa.

Gene ID: 6649

Swiss-Prot: P08294

Immunogen: Human EC SOD purified from aortas

Format: Liquid

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 4GG11G6

Description: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms. There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge. The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space. SOD3 can also be distinguished by its heparin-binding capacity.
Synonyms: EC-SOD, MGC20077, EC 1.15.1.1, SOD3

Specificity: Detects EC-SOD ~35kD, does not cross react with other SODs. Species cross-reactivity: Human, Rat, Mouse, other species not tested.

Sensitivity: 1 µg/mL of SMC-167 was sufficient for detection of EC-SOD in 20µg of human cartilage lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1:1000 (WB), 1:100 (IHC and ICC)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

EC-SOD, (4GG11G6, human cartilage)

EC SOD (4GG11G6), Human cartilage, ICC Left control, middle young, right cartilage with osterarthritis