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Lysosomal-Associated Membrane Protein 2 (LAMP2) antibody

Details for Product No. ABIN361746
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Antigen
Synonyms cd107b, lamp-2, lampb, LAMP2, lamp2, DKFZp459J126, LAMP-2, LGP-B, CD107b, Lamp-2, Lamp-2a, Lamp-2b, Lamp-2c, Mac3, LAMPB, LGP110
Reactivity
Mouse (Murine), Rabbit
(117), (116), (25), (21), (13), (13), (6), (2)
Host
Rat
(83), (82), (52), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(28), (20), (12), (11), (5), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)
(95), (91), (58), (49), (46), (40), (28), (28), (24), (10), (2), (2)
Pubmed 6 references available
Quantity 100 µg
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Catalog No. ABIN361746
345.40 $
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Immunogen Purified preparation of mouse liver lysosomal membranes
Clone GL2A7
Isotype IgG1
Specificity Recommended for use in ICC. The antibody will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin.
Purification Protein G Purified
Alternative Name LAMP2
Background Synonyms:
CD107b, Igp110, Igp2, Lamp2C, LampB, MAC3
Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures,with 37 % sequence homology. They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane.LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cell-extracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis. Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxyl-terminal tyrosine based motif (YXXI). This stimulation has also been shown to have an associated release of histamine, leukotriene C and prostaglandin D, which shows that LAMP1 and LAMP2 are activation markers for normal mast cells. They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation.LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content.
Gene ID 16784
NCBI Accession NP_001017959.1
UniProt P17047
Research Area Stem Cells, Hematopoietic Progenitors, Autophagy, Metabolism, Organelles, Chaperones
Application Notes Recommended Dilution: 1:500- 1:1000 (ICC)
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) antibody LAMP2 (GL2A7), IF showing distribution of lysosomes in CEC Courtesy of Eunduck E P Kay, Doheny Eye Institue.
Background publications Granger, Green, Gabel et al.: "Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells." in: The Journal of biological chemistry, Vol. 265, Issue 20, pp. 12036-43, 1990 (PubMed).

Rohrer, Schweizer, Russell et al.: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, 1996 (PubMed).

Kannan, Stewart, Bounds et al.: "Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium." in: Cellular immunology, Vol. 171, Issue 1, pp. 10-9, 1996 (PubMed).

Lichter-Konecki, Moter, Krawisz et al.: "Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis." in: Differentiation; research in biological diversity, Vol. 65, Issue 1, pp. 43-58, 1999 (PubMed).

Tanaka, Guhde, Suter et al.: "Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice." in: Nature, Vol. 406, Issue 6798, pp. 902-6, 2000 (PubMed).

Grützkau, Smorodchenko, Lippert et al.: "LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 61, Issue 1, pp. 62-8, 2004 (PubMed).

Validation Images
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