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Phosphothreonine antibody

Details for Product No. ABIN361757, Supplier: Log in to see
Antigen
Reactivity
Chemical
78
3
1
Host
Rabbit
62
20
Clonality
Polyclonal
Conjugate
Un-conjugated
9
8
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
76
63
52
18
16
14
11
3
1
1
1
1
Supplier
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Immunogen Phosphothreonine conjugated to KLH
Specificity Detects proteins phosphorylated on threonine residues. Does not cross-react with phosphotyrosine.
Purification Rabbit immunoglobulin
Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine, others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
Research Area Signaling, Metabolism, Amino Acids, Protein Modifications, Cell Signaling, Phosphorylation
Pathways
Application Notes Recommended Dilution: WB (1:500), ICC/IF (1:150), ELISA (1:2000), IP (1:100)
Optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
 image for anti-Phosphothreonine antibody (ABIN361757) Phospho Threonine, mouse brain extract.
Background publications Goto, Kiyono, Tomono et al.: "Complex formation of Plk1 and INCENP required for metaphase-anaphase transition." in: Nature cell biology, Vol. 8, Issue 2, pp. 180-7, 2006 (PubMed).

Downward: "The ins and outs of signalling." in: Nature, Vol. 411, Issue 6839, pp. 759-62, 2001 (PubMed).

Blume-Jensen, Hunter: "Oncogenic kinase signalling." in: Nature, Vol. 411, Issue 6835, pp. 355-65, 2001 (PubMed).

Pawson, Saxton: "Signaling networks--do all roads lead to the same genes?" in: Cell, Vol. 97, Issue 6, pp. 675-8, 1999 (PubMed).

Ostrovsky, Maloy: "Protein phosphorylation on serine, threonine, and tyrosine residues modulates membrane-protein interactions and transcriptional regulation in Salmonella typhimurium." in: Genes & development, Vol. 9, Issue 16, pp. 2034-41, 1995 (PubMed).