CAV3.2 antibody

Antigen: CAV3.2

Clonality: Monoclonal (S55-10)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Catalog no.: ABIN361762

Additional Information

Characteristics: Detects ~260kDa. No cross-reactivity against Cav1.3

Gene ID: 8912

Swiss-Prot: O95180

Immunogen: Fusion protein amino acids 1019-1293 (II-III loop) of human Cav3.2, accession number O95180

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: S55-10

Description: Specifically, Cav3.2 is a protein which in humans is encoded by the CACNA1H gene. Studies suggest certain mutations in this gene lead to childhood absence epilepsy . Studies also suggest that the up-regulations of Cav3.2 may participate in the progression of prostate cancer toward an androgen-independent stage.
Synonyms: CACNA1HB, Alpha1 3.2, CACNA 1H, CACNA1 HB, Cav T.2, EIG6, Voltage dependent T type calcium channel alpha 1H

Specificity: ~260kDa. No cross-reactivity against Cav1.3. Species cross-reactivity: Human.

Sensitivity: 1 µg/mL of SMC-303 was sufficient for detection of Cav3.2 in 10µg of HEK cell lysate expressing Cav3.2 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

Cav3.2 (S55-10), Human hippocampus

Publications

Publications: Chen, Lu, Pan et al.: "Association between genetic variation of CACNA1H and childhood absence epilepsy." in: Annals of neurology, Vol. 54, Issue 2, pp. 239-43, 2003 (PubMed).

Khosravani, Altier, Simms et al.: "Gating effects of mutations in the Cav3.2 T-type calcium channel associated with childhood absence epilepsy." in: The Journal of biological chemistry, Vol. 279, Issue 11, pp. 9681-4, 2004 (PubMed).

Gackiuere, Bidaux, Delcourt et al.: "CaV3.2 T-type calcium channels are involved in calcium-dependent secretion of neuroendocrine prostate cancer cells." in: The Journal of biological chemistry, Vol. 283, Issue 15, pp. 10162-73, 2008 (PubMed).