Transient Receptor Potential Cation Channel, Subfamily V, Member 3 (TRPV3) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 3 (TRPV3)

Synonyms: VRL3, Nh, AI644701, MGC124324, MGC124325, 1110036I10Rik, TRPV3, MGC159979

Clonality: Monoclonal (S15-4)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Catalog no.: ABIN361778

Additional Information

Characteristics: Detects ~70kDa

Alternative name: TrpV3

Gene ID: 497948

Immunogen: Synthetic peptdie amino acids 458-474 (C-terminus) of rat TrpV3, accession number NP_001020928

Format: Liquid

Isotype: IgG2a  (Matching secondary antibodies)

Clone: S15-4

Description: The TRPV3 protein belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. The gene lies in close proximity to another family member (TRPV1) gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels .
Synonyms: transient receptor potential cation channel subfamily V member 3, VRL3

Specificity: ~70kDa. Species cross-reactivity: Human, Mouse, Rat.

Sensitivity: 1 µg/mL of SMC-319 was sufficient for detection of TrpV3 in 10µg of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

TrpV3 (S15-4), Human Cell line mix

TrpV3 (S15-4), Human Hippocampus

Publications

Publications: Xiao, Tang, Wang et al.: "Calcium plays a central role in the sensitization of TRPV3 channel to repetitive stimulations." in: The Journal of biological chemistry, Vol. 283, Issue 10, pp. 6162-74, 2008 (PubMed).

Masamoto, Kawabata, Fushiki: "Intragastric administration of TRPV1, TRPV3, TRPM8, and TRPA1 agonists modulates autonomic thermoregulation in different manners in mice." in: Bioscience, biotechnology, and biochemistry, Vol. 73, Issue 5, pp. 1021-7, 2009 (PubMed).