Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4) antibody

Antigen: Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4)

Synonyms: HCN4, hcn4

Clonality: Monoclonal (S114-10)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Catalog no.: ABIN361779

Additional Information

Characteristics: Detects ~130kDa. No cross-reactivity against other HCNs

Alternative name: HCN4

Gene ID: 59266

Swiss-Prot: Q9JKA7

Immunogen: Fusion protein amino acids 1-019-1198 (c-terminus) of rat HCN4, accession number Q9JKA7

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: S114-10

Description: Hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain.
Synonyms: hyperpolarization activated cyclic nucleotide gated potassium channel 4

Specificity: ~130kDa. No cross reactivity against other HCNs. Species cross-reactivity: Mouse, Rat, Human.

Sensitivity: 1 µg/mL of SMC-320 was sufficient for detection of HCN4 in 10µg of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details

Application Notes: 1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS pH7.4, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Restrictions: For Research Use only

Images

HCN4 (S114-10), Human Hippocampus

HCN4 (S114-10), T-HEK, induced

Publications

Publications: Zong, Eckert, Yuan et al.: "A novel mechanism of modulation of hyperpolarization-activated cyclic nucleotide-gated channels by Src kinase." in: The Journal of biological chemistry, Vol. 280, Issue 40, pp. 34224-32, 2005 (PubMed).