FKBP5 antibody

Catalog No. ABIN361795

This Mouse Monoclonal antibody specifically detects FKBP5 in WB, IF and ICC. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for FKBP5 antibody (ABIN361795)

Target: FKBP5 (FK506 Binding Protein 5 (FKBP5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FKBP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: Hi51B

Product Details anti-FKBP5 Antibody

Specificity: Detects ~51 kDa.

Cross-Reactivity: Dog, Hamster, Human, Mouse, Rabbit, Rat

Purification: Protein G Purified

Immunogen: Synthetic peptide corresponding to the residues of human FKBP51

Isotype: IgG1

Application Details

Application Notes:

• WB (1:2000)
• ICC/IF (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:2000 dilution was sufficient for detection of FKBP51 in ~50 μg total protein using WB analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-FKBP5 antibody (ABIN361795)

Mata-Greenwood, Jackson, Pearce, Zhang: "Endothelial glucocorticoid receptor promoter methylation according to dexamethasone sensitivity." in: Journal of molecular endocrinology, (2015) (PubMed).

Target Details for FKBP5

Target: FKBP5 (FK506 Binding Protein 5 (FKBP5))

Alternative Name: FKBP51

Background: HSP90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones (1). One class of HSP90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an HSP90 binding site. Among the TPR cochaperones of HSP90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins (2). FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex (3). The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the HSP90 (4). Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different HSP90-binding characteristics (3). FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids (5).

Gene ID: 2289

NCBI Accession: NP_001139247

UniProt: Q13451