FK506 Binding Protein 5 (FKBP5) antibody

Antigen: FK506 Binding Protein 5 (FKBP5)

Synonyms: P54, AIG6, FKBP51, FKBP54, PPIase, Ptg-10, MGC111006, Dit1, 51kDa, D17Ertd592e, zgc:64082, wu:fc31g11, wu:fl87b03, si:zc263a23.8, FKBP5, DKFZp469O2021

Clonality: Monoclonal (Hi51B)

Host: Mouse

Reactivity: Dog (Canine), Hamster, Human, Mouse (Murine), Rat (Rattus), Rabbit

Application: Western Blotting (WB)

Catalog no.: ABIN361795

Additional Information

Characteristics: Detects an ~51kDa protein representing FKBP51 in cell lysate. Also detects FKBP51 in whole tissue extracts from rat kidney and rat and mouse testes.

Alternative name: FKBP51

Gene ID: 2289

Swiss-Prot: Q13451

Immunogen: Synthetic peptide corresponding to the residues of human FKBP51

Format: Liquid

Isotype: IgG  (Matching secondary antibodies)

Clone: Hi51B

Description: Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones. One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins . FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex. The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90. Although FKBP52 and FKBP51 share ~75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics. FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids.
Synonyms: AIG6, FK506 binding protein 5, FKBP5, FKBP54, Hsp90 binding immunophilin, p54, Pplase, Ptg10, Rotamase, T cekk FK506 binding protein antibody

Sensitivity: A 1:2000 dilution was sufficient for detection of FKBP51 in ~50µg total protein using WB anaylsis.

Application Details

Application Notes: 1:2000 (WB)

Concentration: 1 mg/mL

Purification: Protein G Purified

Buffer: PBS, 50% glycerol

Storage: -20 °C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Signaling

Restrictions: For Research Use only

Images

FKBP51 (Hi51B), IF localization in normal MK cells shown in red. Courtesy of the Hospital Henri Mondor, France