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Acetylated Lysine (acLys) antibody

Antigen

Acetylated Lysine
Binding Site
Alternatives

acLys
Clonality Monoclonal (7F8)
Host
Alternatives

Mouse
Reactivity
Alternatives

Cow (Bovine), Bird (Avian)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN361802
Quantity
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anti-Acetylated Lysine (acLys) antibody

Additional Information
Application Details Publications Images Alternatives Variants back to top

Immunogen Acetylated KLH
Isotype IgG1
Clone 7F8
Description Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs). Protein posttranslational reversible lysine Nepsilon development.

Synonyms: lysine, acetyl lysine
Specificity Detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.
Sensitivity 1µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Application Details
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Concentration 1mg/mL
Purification Protein G purified
Buffer PBS pH7.4, 50% glycerol
Storage Store at -20° C. Shipping Conditions: Blue Ice or 4° C
Storage Shipping Temp Max Blue Ice or 4 °C
Research Area Cell Signaling
Restrictions For Research Use only

Publications
Application Details Images Alternatives Variants back to top

Hughes: "Polyglutamine disease: acetyltransferases awry." in: Current biology : CB, Vol. 12, Issue 4, pp. R141-3, 2002 (PubMed).

Vigushin, Coombes: "Targeted histone deacetylase inhibition for cancer therapy." in: Current cancer drug targets, Vol. 4, Issue 2, pp. 205-18, 2004 (PubMed).

Yang: "Lysine acetylation and the bromodomain: a new partnership for signaling." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 26, Issue 10, pp. 1076-87, 2004 (PubMed).

Yang: "Multisite protein modification and intramolecular signaling." in: Oncogene, Vol. 24, Issue 10, pp. 1653-62, 2005 (PubMed).

Alternatives
Application Details Publications Images Variants back to top

Alternatives for antigen "Acetylated Lysine", type "Antibodies"
Hosts Mouse (13), Rabbit (13)
Reactivities Bird (Avian) (5), Cow (Bovine) (4), All Species (3), Chicken (1), Human (1), Mammalian (1)
Applications Western Blotting (WB) (26), ELISA (22), Immunoprecipitation (IP) (9), Immunofluorescence (IF) (8), Immunohistochemistry (IHC) (6), Immunocytochemistry (ICC) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Enzyme Immunoassay (EIA) (1)
Conjugates Biotin (4), HRP (4)
Epitopes acLys (8)