»
Antibody
»
anti-Acetylated Lysine
Acetylated Lysine antibody
| Antigen |
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| Clonality |
Monoclonal (7F8) |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
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| Catalog no. |
ABIN361803 |
| Quantity |
100ug (1.0mg/mL) (Variants) |
| Price |
325.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 10 Business Days |
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Immunogen
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Acetylated KLH
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Format
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Protein G purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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7F8
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Description
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Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Ne-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3). The regulation of protein acetylation status is impaired in the pathologies of cancer and polyglutamine diseases (4), and HDACs have become promising targets for anticancer drugs currently in development (5).
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Specificity
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Detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues. Species cross-reactivity: Bovine, Avian. Not otherwise tested.
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Concentration
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1.0mg/mL
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Buffer
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Protein G purified. Mouse immunoglobulin in PBS, pH 7.4, 0.09% sodium azide in 50% glycerol
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Storage
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-20°C, 1 year+, shipped on cold packs or ambient
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Restrictions
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For Research Use only
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Alternatives for antigen "Acetylated Lysine", type "Antibodies"
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Hosts
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Rabbit (16), Mouse (8)
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Reactivities
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Bird (Avian) (5), Cow (Bovine) (3), Mammalian (2), All Species (1), Human (1), Various Species (1)
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Applications
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Western Blotting (WB) (23), ELISA (13), Immunoprecipitation (IP) (11), Immunofluorescence (IF) (9), Enzyme Immunoassay (EIA) (8), Immunohistochemistry (IHC) (7), Flow Cytometry (FACS) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Radioimmunoassay (RIA) (1)
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Conjugates
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Biotin (4), HRP (4)
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