Ubiquitin antibody

Antigen: Ubiquitin

Synonyms: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Clonality: Monoclonal (6C11-B3)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN361816

Additional Information

Gene ID: 7314

UniProt: P62988

Immunogen: Native bovine ubiquitin, conjugated to KLH

Isotype: IgG2a kappa

Clone: 6C11-B3

Description: Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity. The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome. Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(2, 3). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated. Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkappaBalpha and p27.

Synonyms: Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B

Characteristics: Accession Number: NP_061828.1

Specificity: This antibody recognizes ~10kDa corresponding to free ubiquitin

Sensitivity: 1 µg/mL of SMC-171 was sufficient for detection of ubiquitin in 10µg of Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Synonyms: MGC8385, FLJ25987, Ubc, Ubb2, Uba52, Rps27a, AL033289, CG11624, DmelCG11624, DmUb, Ub, ubi, Ubi-p, ubi-p63E, Ubi-P63e, ubi63E, Ubi63E, Ubi63p, Ubip, Ubiq, Ubp, UBC, CG18282, CG32744, CR32744, DmelCG32744, ubiquitin, si:dz180g5.5, si:busm1-180g5.5, UBB, Ubiquitin, CG11700, ESTS:143G11T, ESTS:199A6T, anon-sts41, poly-ub, DmelCR11700, LOC100194618, UBIQ, LOC100282068

Application Details

Application Notes: 1:1000 (WB)

Concentration: 1mg/mL

Purification: Protein G purified

Buffer: PBS pH7.4, 50% glycerol

Storage: Store at -20° C. Shipping Conditions: Blue Ice or 4° C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Cell Signaling, Neurology, Proteolysis / Ubiquitin, Alzheimer's Disease, Proteases, Amino Acids

Restrictions: For Research Use only

Images

SMC 171, Ubiquitin (6C11 B3), human cvell line mix copy.

Publications

Wilkinson: "Roles of ubiquitinylation in proteolysis and cellular regulation." in: Annual review of nutrition, Vol. 15, pp. 161-89, 1996 (PubMed).

Chen, Parent, Maniatis: "Site-specific phosphorylation of IkappaBalpha by a novel ubiquitination-dependent protein kinase activity." in: Cell, Vol. 84, Issue 6, pp. 853-62, 1996 (PubMed).

Bonifacino, Weissman: "Ubiquitin and the control of protein fate in the secretory and endocytic pathways." in: Annual review of cell and developmental biology, Vol. 14, pp. 19-57, 1999 (PubMed).