CRYAB antibody

Catalog No. ABIN361837

This anti-CRYAB antibody is a Rabbit Polyclonal antibody detecting CRYAB in WB, IHC, IF and ICC. Suitable for Human.

Quick Overview for CRYAB antibody (ABIN361837)

Target: CRYAB (Crystallin, alpha B (CRYAB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRYAB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CRYAB Antibody

Specificity: Detects ~22 kDa. Does not cross-react with αA-crystallin.

Cross-Reactivity: Chicken, Cow, Human, Mouse, Rat

Purification: Affinity Purified

Immunogen: Synthetic peptide corresponding to human alpha B crystallin conjugated to KLH

Application Details

Application Notes:

• WB (1:5000)
• ICC/IF (1:120)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:5000 dilution of ABIN361836 was sufficient for detection of alpha B crystallin in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for CRYAB

Target: CRYAB (Crystallin, alpha B (CRYAB))

Alternative Name: Alpha B Crystallin

Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).

Gene ID: 1410

NCBI Accession: NP_001876

UniProt: P02511