Heat Shock 22kDa Protein 8 (HSPB8) antibody

Antigen: Heat Shock 22kDa Protein 8 (HSPB8)

Synonyms: H11, HMN2, CMT2L, DHMN2, E2IG1, HMN2A, HSP22, MGC64408, HSPB8, DKFZp468F234, fc09c11, MGC64202, zgc:64202, wu:fc04b04, wu:fc09c11

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN361851

Additional Information

Alternative name: Hsp22

Gene ID: 26353

UniProt: Q9UJY1

Immunogen: Human Hsp22

Description: Hsp22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to Hsp27. Similar to most other sHSPs, Hsp22 is predominately transcribed in skeletal muscle and heart, as well as the placenta. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HspB8 interacted preferentially with a triple aspartate form of Hsp27 which mimics Hsp27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type Hsp27. HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs. The chaperone-like activity is of great importance to the function of Hsp22 in various processes including proliferation, apoptosis and macroautophagy. Mutations in the HSPB8 gene are associated with the inherited peripheral neurpathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).
Synonyms: Alpha crystallin C chain, CMT2L, CRYAC, DHMN2, H11, Heat shock 22kDa protein 8, HMN2, HSB8, HspB8

Characteristics: Accession Number: NP_055180.1

Specificity: Detects ~22kDa. Does not cross-react with Hsp27 or alpha-crystallin.

Application Details

Application Notes: 1:1000 (WB)

Purification: Rabbit antiserum

Storage: Store at -20° C. Shipping Temperature: Blue Ice or 4° C

Storage Shipping Temp Max: Blue Ice or 4 °C

Research Area: Heat Shock Proteins, Neurology

Restrictions: For Research Use only

Images

Hsp22, Mouse Spinal Cord

Publications

Benndorf, Sun, Gilmont et al.: "HSP22, a new member of the small heat shock protein superfamily, interacts with mimic of phosphorylated HSP27 ((3D)HSP27)." in: The Journal of biological chemistry, Vol. 276, Issue 29, pp. 26753-61, 2001 (PubMed).

Kappé, Verschuure, Philipsen et al.: "Characterization of two novel human small heat shock proteins: protein kinase-related HspB8 and testis-specific HspB9." in: Biochimica et biophysica acta, Vol. 1520, Issue 1, pp. 1-6, 2001 (PubMed).

Sun, Fontaine, Rest et al.: "Interaction of human HSP22 (HSPB8) with other small heat shock proteins." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2394-402, 2004 (PubMed).

Kim, Seit-Nebi, Gusev: "The problem of protein kinase activity of small heat shock protein Hsp22 (H11 or HspB8)." in: Biochemical and biophysical research communications, Vol. 325, Issue 3, pp. 649-52, 2004 (PubMed).

Wilhelmus, Boelens, Otte-Hoeller et al.: "Small heat shock protein HspB8: its distribution in Alzheimer's disease brains and its inhibition of amyloid-beta protein aggregation and cerebrovascular amyloid-beta toxicity." in: Acta neuropathologica, Vol. 111, Issue 2, pp. 139-49, 2006 (PubMed).