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LIM Domain Kinase 2 (LIMK2) (pThr505) antibody

Details for Product No. ABIN362109, Supplier: Login to see
Antigen
Epitope
pThr505
28
15
10
9
7
6
6
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
127
62
59
1
Host
Rabbit
115
7
3
2
Clonality
Polyclonal
Conjugate
Un-conjugated
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
109
54
44
39
13
10
2
1
1
1
1
1
1
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Immunogen Peptide sequence around phosphorylation site of pThr505 (R-Y-T (p) -V-V) derived from Human LIMK2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of LIMK2 only when phosphorylated at threonine 505.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name LIMK2 (LIMK2 Antibody Abstract)
Background Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Molecular Weight 70 kDa
NCBI Accession NP_001026
UniProt P53671
Pathways
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Ohashi, Nagata, Maekawa et al.: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

Edwards, Sanders, Bokoch et al.: "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics." in: Nature cell biology, Vol. 1, Issue 5, pp. 253-9, 1999 (PubMed).