HDAC5 antibody (pSer498)

Catalog No. ABIN362217

Cited in 6+ publications. The Rabbit Polyclonal anti-HDAC5 antibody (ABIN362217) specifically detects HDAC5 in WB and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HDAC5 antibody (pSer498) (ABIN362217)

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HDAC5 Antibody

Binding Specificity: pSer498

Specificity: The antibody detects endogenous level of HDAC5 only when phosphorylated at serine498.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer498 (T-Q-S (p) -S-P) derived from Human HDAC5, 7. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100  

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-HDAC5 antibody (ABIN362217)

Wang, Ha, Jhun, Wong, Jain, Jin: "Fluid shear stress stimulates phosphorylation-dependent nuclear export of HDAC5 and mediates expression of KLF2 and eNOS." in: Blood, Vol. 115, Issue 14, pp. 2971-9, (2010) (PubMed).

Xia, Li, Johnson, Seidel, Wallace, Li: "Polycystin-dependent fluid flow sensing targets histone deacetylase 5 to prevent the development of renal cysts." in: Development (Cambridge, England), Vol. 137, Issue 7, pp. 1075-84, (2010) (PubMed).

Borok, Tran, Ho, Drewell: "Dissecting the regulatory switches of development: lessons from enhancer evolution in Drosophila." in: Development (Cambridge, England), Vol. 137, Issue 1, pp. 5-13, (2009) (PubMed).

Bossuyt, Helmstadter, Wu, Clements-Jewery, Haworth, Avkiran, Martin, Pogwizd, Bers: "Ca2+/calmodulin-dependent protein kinase IIdelta and protein kinase D overexpression reinforce the histone deacetylase 5 redistribution in heart failure." in: Circulation research, Vol. 102, Issue 6, pp. 695-702, (2008) (PubMed).

Pang, Yan, Natarajan, Cavet, Massett, Yin, Berk: "GIT1 mediates HDAC5 activation by angiotensin II in vascular smooth muscle cells." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, Issue 5, pp. 892-8, (2008) (PubMed).

Lim, Luo, Koh, Yang, bin Abdul Kadir, Tan, Ye, Wang, Melamed et al.: "Distinct mechanisms involving diverse histone deacetylases repress expression of the two gonadotropin beta-subunit genes in immature gonadotropes, and their actions are overcome by ..." in: Molecular and cellular biology, Vol. 27, Issue 11, pp. 4105-20, (2007) (PubMed).

Target Details for HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Alternative Name: HDAC5

Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene. Doppler H, et al. (2005) J Biol Chem. 280(15):15013-15019. McKinsey TA, et al. (2000) Nature. 408(6808): 106-111.   Published Papers J Bossuyt, K Helmstadter, X Wu, et al. Ca2+/Calmodulin-Dependent Protein Kinase II {delta} and Protein Kinase D Overexpression Reinforce. Circulation Research, 102(6):695-702.(2008) Pang J, Yan C, Natarajan K, et al. GIT1 mediates HDAC5 activation by angiotensin II in vascular smooth muscle cells.Arteriosclerosis, Thrombosis, and Vascular Biology, 28(5):892-8. (2008) S Lim, M Luo, M Koh, et al. Distinct Mechanisms Involving Diverse Histone Deacetylases Repress Expression of the Two Gonadotropin beta-Subunit Genes in Immature Gonadotropes, and Their Actions Are Overcome by Gonadotropin-Releasing Hormone.

Molecular Weight: 124 kDa

NCBI Accession: NP_001015

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process