EIF4G1 antibody (AA 1230-1234)

Catalog No. ABIN363083

The Rabbit Polyclonal anti-EIF4G1 antibody is suitable to detect EIF4G1 in samples from Human. It has been validated for IHC, WB and IF.

Quick Overview for EIF4G1 antibody (AA 1230-1234) (ABIN363083)

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4G1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-EIF4G1 Antibody

Binding Specificity: AA 1230-1234

Specificity: The antibody detects endogenous level of total eIF4G protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen. Specificity/Sensitivity eIF4G (Ab-1232) Antibody detects endogenous levels of total eIF4G protein.

Immunogen: Peptide sequence around AA 1230-1234 (P-V-S-P-L) derived from Human eIF4G. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for EIF4G1

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Alternative Name: eIF4G

Background: EIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E, insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3, in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis

Molecular Weight: 220 kDa

NCBI Accession: NP_004944

UniProt: Q04637