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Rabbit anti-Human IgG Antibody (HRP)

Antigen

IgG

Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human

Conjugate
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Catalog no. ABIN363131
Quantity 1ml  (1ml)
Price 185.00 $   Plus shipping costs $35.00
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Additional Information

Immunogen The antiserum was developed in Rabbit using the human IgG as the immunogen.
Isotype IgG
Specificity Rabbit anti-Human IgG conjugate is specific for human IgG. No cross-reactivity was observed with human IgA, IgM proteins by ELISA.

Application Details

Application Notes ELISA : 1:5000~1:20000, WB : 1:5000~1:10000, IHC 1:1000~5000
Concentration 1ml
Purification The antibody is obtained by affinity purified technique, which removes essentially all rabbit serum proteins, except the specific antibody for human IgG. Rabbit anti-human IgG is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK.
Buffer HRP-labeled Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl.
Storage Store at 2-8°C. Avoid repeat freeze-thaw cycles.
Restrictions For Research Use only

Alternatives

Alternatives for antigen "IgG", type "Secondary Antibodies"
Hosts Goat (1061), Rabbit (610), Donkey (491), Chicken (186), Sheep (78), Mouse (65), Rat (9), Duck (6), Guinea Pig (6), Horse (4)
Reactivities Rabbit (517), Mouse (Murine) (451), Human (406), Goat (293), Rat (Rattus) (253), Guinea Pig (141), Sheep (Ovine) (112), Hamster (63), Cow (Bovine) (45), Chicken (41), Syrian Hamster (37), Cat (Feline) (36), Dog (Canine) (33), Llama (30), Donkey (25), Horse (Equine) (14), Monkey (13), Pig (Porcine) (5), Bird (Avian) (3), Armenian Hamster (1)
Conjugates HRP (303), FITC (244), Biotin (231), Alkaline Phosphatase (AP) (212), DyLight 488 (141), TRITC (136), Texas Red (TR) (108), DyLight 405 (87), DyLight 594 (87), DyLight 680 (87), DyLight 800 (87), Rhodamine (64), PE (19), Beads (18), 488-NHS Ester (13), DyLight 350 (13), DyLight 550 (13), DyLight 633 (13), DyLight 650 (13), RPE (12), DyLight 549 (6), Agarose Beads (5), Colloidal Gold (10nm) (5), Cy5 (5), Colloidal Gold (5nm) (4), Cy3 (4), APC (3), SPRD (3), beta-Gal (3), AMCA (2), Colloidal Gold (20nm) (2), Cy2 (2), Poly-HRP (2), Alexa Fluor 647 (1), Colloidal Gold (15nm) (1), FL (1), PE,RPE (1)
Epitopes H&L (1368), Fc specific (73), Fab (1)