MAP1LC3A antibody (N-Term)

Catalog No. ABIN363515

The Rabbit Polyclonal anti-MAP1LC3A antibody is suitable to detect MAP1LC3A in samples from Human, Mouse, Rat, Zebrafish (Danio rerio), Cow and Bacteria. It has been validated for WB, IHC, IF, ICC and IP.

Quick Overview for MAP1LC3A antibody (N-Term) (ABIN363515)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Bacteria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-MAP1LC3A Antibody

Binding Specificity: AA 1-100, N-Term

Specificity: Reacts withLC3 from human, mouse, rat, bacteria, golden syrian hamster, porcine and zebrafish

Purification: Antigen affinity purified

Immunogen: Synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100)

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
WB0.5-2 μg/mL ICC/IF1:200 IHC1:200-1:400 IP20 μg/500 μg of protein

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Tris-glycine, 150 mM NaCl, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: Light Chain 3 (LC3)

Background: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Gene ID: 81631

Pathways: Autophagy