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Mouse anti-Human IgE (Fc Region) Antibody

ELISA, WB, FACS, Func Monoclonal BSW17-NBS01 IgG1 kappa unconjugated
Catalog No. ABIN363704
  • Target See all IgE products
    IgE
    Binding Specificity
    • 30
    • 19
    • 17
    • 1
    • 1
    Fc Region
    Reactivity
    • 48
    • 30
    • 10
    • 10
    • 5
    • 4
    • 2
    • 1
    Human
    Host
    • 58
    • 18
    • 10
    • 10
    • 4
    • 1
    Mouse
    Clonality
    • 66
    • 28
    Monoclonal
    Application
    • 55
    • 39
    • 30
    • 20
    • 20
    • 15
    • 12
    • 10
    • 10
    • 8
    • 8
    • 6
    • 5
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
    Specificity
    Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
    Characteristics
    IGHE, chromosome 14q32.33
    Purification
    Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
    Purity
    > 95 %, tested by analytical Gel Permeation Chromatography
    Immunogen
    purified human myeloma IgE
    Clone
    BSW17-NBS01
    Isotype
    IgG1 kappa
  • Application Notes
    When used for functional cellular assays (mast cell / basophil activation) at dilutions < 1:100, the Na-azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS, pH=7.2, 0.1 % NaN3
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
    Storage
    4 °C
  • Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).

  • Target
    IgE
    Abstract
    IgE Products
    Background
    The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
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