CASZ1 antibody (N-Term)

Catalog No. ABIN364232

The Rabbit Polyclonal anti-CASZ1 antibody is suitable to detect CASZ1 in samples from Human, Mouse, Rat and Cow. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for CASZ1 antibody (N-Term) (ABIN364232)

Target: CASZ1 (Castor Zinc Finger 1 (CASZ1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASZ1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CASZ1 Antibody

Binding Specificity: AA 1-150, N-Term

Specificity: Human Cas5 protein. A BLAST analysis was used to suggest cross reactivity with Cas5 from mouse, chimpanzee, bovine, and rat based on 100 % homology with the immunizing sequence. Expect cross reactivity with multiple isoforms of this protein. Cross reactivity with Cas5 from other sources has not been determined

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between amino acids 1-150 of B3KRV8

Application Details

Application Notes: Approved: ELISA (1:25000 - 1:100000), IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: A band approximately 125 kD in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CASZ1

Target: CASZ1 (Castor Zinc Finger 1 (CASZ1))

Alternative Name: CASZ1

Background: Name/Gene ID: CASZ1

Synonyms: CASZ1, Castor-related protein, CAS11, Castor, Castor homolog 1, zinc finger, CST, DJ734G22.1, Zinc finger protein 693, SRG, ZNF693, Castor zinc finger 1, Survival-related

Gene ID: 54897

UniProt: Q86V15