CEBPB antibody

Catalog No. ABIN364320

The Mouse Monoclonal anti-CEBPB antibody (Clone 47A1) (ABIN364320) specifically detects CEBPB in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for CEBPB antibody (ABIN364320)

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEBPB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 47A1

Product Details anti-CEBPB Antibody

Immunogen: Recombinant human C/EBP-kappa(1-271 aa) purified from E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (1:500 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details for CEBPB

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Alternative Name: C/EBP Beta / CEBPB

Background: Name/Gene ID: CEBPB

Synonyms: CEBPB, C/EBP-beta, C/EBP beta, CRP2, LAP, Liver activator protein, Nuclear factor NF-IL6, IL6DBP, TCF5, NFIL6, TCF-5, Transcription factor 5, NF-IL6

Gene ID: 1051

UniProt: P17676

Pathways: Interferon-gamma Pathway, Autophagy, Brown Fat Cell Differentiation