PSMA antibody

Catalog No. ABIN364431

The Mouse Monoclonal anti-PSMA antibody (Clone Y-PSMA1) (ABIN364431) specifically detects PSMA in WB, IHC, ELISA, IHC (p), IF and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for PSMA antibody (ABIN364431)

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PSMA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: Y-PSMA1

Product Details anti-PSMA Antibody

Specificity: Recognizes PSMA expressed in LNCap cell lines. Little or no cross-reactivity to benign prostate hyperplasia (BPH) or to normal prostatic tissue.

Purification: Protein G purified

Immunogen: Crude membrane protein preparation from pooled prostate malignant carcinoma from China.

Type of Immunogen: Membrane extract

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.0, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PSMA

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Alternative Name: FOLH1 / PSMA

Background: Name/Gene ID: FOLH1
Subfamily: Metallopeptidase M28B

Synonyms: FOLH1, Folate hydrolase 1, GCPII, Glutamate carboxypeptidase II, Folate hydrolase, GCP2, NAALAD1, NAALAdase, NAALADase I, PSM, FGCP, FOLH, Glutamate carboxylase II, Glutamate carboxypeptidase 2, MGCP, PSMA

Gene ID: 2346

UniProt: Q04609