MAT2A antibody (AA 1-395)

Catalog No. ABIN364446

The Mouse Monoclonal anti-MAT2A antibody (Clone AT3A2) (ABIN364446) specifically detects MAT2A in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for MAT2A antibody (AA 1-395) (ABIN364446)

Target: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAT2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AT3A2

Product Details anti-MAT2A Antibody

Binding Specificity: AA 1-395

Purification: Protein G purified

Immunogen: Recombinant human MAT2A (1-395aa) purified from E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for MAT2A

Target: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Alternative Name: MAT2A

Background: Name/Gene ID: MAT2A

Synonyms: MAT2A, AdoMet synthase 2, AMS2, AdoMet synthetase 2, MATA2, MATII, MAT 2, MAT-II, SAMS2

Gene ID: 4144

UniProt: P31153

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process