T-Cell Receptor (TCR), gamma/delta- antibody (PE)

Antigen: T-Cell Receptor (TCR), gamma/delta-

Clonality: Monoclonal (UC7-13D5)

Host: Hamster

Reactivity: Mouse (Murine)

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN371053

Additional Information

Characteristics: Synonyms: TCRG, TCRD, T-Cell Receptor gamma, T-Cell Receptor delta, T-Cell Receptor gamma delta

Alternative name: T Cell Receptor (TCR) gamma/delta

Cross-Reactivity: Mouse (Murine)

Isotype: IgG

Clone: UC7-13D5

Description: T cell receptors (TCR) recognize foreign antigens which have been processed as smallpeptides and bound to major histocompatibility complex (MHC) molecules at the surfaceof antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alphaand one beta chain or one delta and one gamma chain. This region represents the germlineorganization of the T cell receptor beta locus. The beta locus includes V (variable), J(joining), diversity (D), and C (constant) segments. During T cell development, the betachain is synthesized by a recombination event at the DNA level joining a D segment with a Jsegment, a V segment is then joined to the D-J gene. The C segment is later joined bysplicing at the RNA level. The TCR gamma/delta heterodimer of the CD3/T-cell receptor complex is clonotypic andconsists of Ig-like variable and constant domains. (Ref. 1) It is expressed duringthymopoiesis and on the small subpopulation of gamma/delta TCR expressing Tlymphocytes in the periphery. The majority of T cells present in some epithelial tissues aregamma/delta+ and have limited receptor diversity. (Ref. 1,2) The TCR gamma/deltaheterodimer recognizes peptide antigen bound to MHC antigens, and subsequent signaltransduction mediated by the invariant chains leads to T cell activation. (Ref. 3)

Specificity: This Monoclonal antibody is specific to the gamma-delta heterodimer and plate-boundUC7-13D5 activates gamma-delta TCR-bearing cells. (Ref. 4-7)It does not react with alpha-beta TCR-expressing T cells. Species: Mouse. Others not tested.

Application Details

Application Notes: Flow Cytometry: < / = 0.3 µg/10e6 cells. (Ref.4-7)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS containing 0.09% Sodium Azide as preservative and a stabilizingagent.Label: R-Phycoerythrin (PE)

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Immunology, Receptors, Signaling

Restrictions: For Research Use only