MHC Class II I-Ab antibody

Catalog No. ABIN371141

The Rat Monoclonal anti-MHC Class II I-Ab antibody is suitable to detect MHC Class II I-Ab in samples from Mouse. It has been validated for FACS and Func.

Quick Overview for MHC Class II I-Ab antibody (ABIN371141)

Target: MHC Class II I-Ab

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MHC Class II I-Ab antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: NIMR-4

Product Details anti-MHC Class II I-Ab Antibody

Characteristics: Synonyms: H2-Eb1, H-2 class II histocompatibility antigen I-A beta chain

Purification: Purified

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. Complement-dependent Cytotoxicity Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MHC Class II I-Ab

Target: MHC Class II I-Ab

Background: MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.Synonyms: H-2 class II histocompatibility antigen I-A beta chain, H2-Eb1

UniProt: P18468