MHC Class I H-2Db antibody

Catalog No. ABIN371165

The Mouse Monoclonal anti-MHC Class I H-2Db antibody is suitable to detect MHC Class I H-2Db in samples from Mouse. It has been validated for FACS.

Quick Overview for MHC Class I H-2Db antibody (ABIN371165)

Target: MHC Class I H-2Db

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS)

Clone: 28-14-8

Product Details

Specificity: This antibody is specific to an epitope in the alpha 3 domain of H-2Db. It binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin. (Ref.7,8) It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, (Ref.9-11) but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. (Ref.12). Customer feedback: Clone 28-14-8 does not cross-react with human cells.

Characteristics: Synonyms: H-2D(B), H2-D1, H-2 class I histocompatibility antigen, D-B alpha chain

Endotoxin Level: Low Endotoxin

Immunogen: C3H. SW mouse splenocytes. (Ref. 1,2)

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. Immunohistochemistry on Frozen Tissue Sections. Complement-mediated cytotoxicity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: MHC Class I H-2Db

Alternative Name: MHC Class I H-2 Db

Background: The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.5)Synonyms: D-B alpha chain, H-2 class I histocompatibility antigen, H-2D(B), H2-D1

Gene ID: 14964

UniProt: P01899