MHC Class I H-2Db antibody

Catalog No. ABIN371166

Cited in 7+ publications. The Mouse Monoclonal anti-MHC Class I H-2Db antibody (Clone 28-14-8) (ABIN371166) specifically detects MHC Class I H-2Db in FACS, CyTox and IP. The antibody is reactive with Mouse samples.

Quick Overview for MHC Class I H-2Db antibody (ABIN371166)

Target: MHC Class I H-2Db

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Cytotoxicity Test (CyTox), Immunoprecipitation (IP)

Clone: 28-14-8

Product Details

Specificity: This antibody is specific to an epitope in the alpha 3 domain of H-2Db. It binds to the alpha 3 domain of H-2Db in the presence or absence of beta 2 microglobulin. (Ref.7,8) It cross reacts with the alpha 3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. The antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, (Ref.9-11) but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. (Ref.12). Customer feedback: Clone 28-14-8 does not cross-react with human cells.

Characteristics: Synonyms: H-2D(B), H2-D1, H-2 class I histocompatibility antigen, D-B alpha chain

Purification: Purified

Immunogen: C3H. SW mouse splenocytes. (Ref. 1,2)

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: < / = 1 μg/10^6 cells. Immunohistochemistry on Frozen Tissue Sections. Complement-mediated cytotoxicity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

References

Lee, Bar-Haim, Machlenkin, Goldberger, Volovitz, Vadai, Tzehoval, Eisenbach: "In vivo rejection of tumor cells dependent on CD8 cells that kill independently of perforin and FasL." in: Cancer gene therapy, Vol. 11, Issue 3, pp. 237-48, (2004) (PubMed).

Wang, Rogers, Ratliff, Russell: "CD95-dependent bystander lysis caused by CD4+ T helper 1 effectors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2961-8, (1996) (PubMed).

Levitsky, Lazenby, Hayashi, Pardoll: "In vivo priming of two distinct antitumor effector populations: the role of MHC class I expression." in: The Journal of experimental medicine, Vol. 179, Issue 4, pp. 1215-24, (1994) (PubMed).

Lie, Myers, Connolly, Gorka, Lee, Hansen: "The specific binding of peptide ligand to Ld class I major histocompatibility complex molecules determines their antigenic structure." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 449-59, (1991) (PubMed).

Allen, Fraser, Flyer, Calvin, Flavell: "Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 19, pp. 7447-51, (1986) (PubMed).

Woodward, Orn, Harmon, Goodenow, Hood, Frelinger: "Specific recognition of the product of a transferred major histocompatibility complex gene by cytotoxic T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 11, pp. 3613-7, (1982) (PubMed).

Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, (1981) (PubMed).

Target Details

Target: MHC Class I H-2Db

Alternative Name: MHC Class I H-2 Db

Background: The 'classical' MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-Microglobulin. (Ref.3,4) These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. (Ref.5)Synonyms: D-B alpha chain, H-2 class I histocompatibility antigen, H-2D(B), H2-D1

Gene ID: 14964

UniProt: P01899