Flow Cytometry. Immunohistochemistry (Acetone-Fixed, Frozen Sections only). (Ref.2)Complement-mediated Cytotoxicity. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
H-2Kk
(MHC, Class I H-2Kk)
Alternative Name
MHC Class I H-2 Kk
Background
MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta2- microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. Lymphocytes are highly positive whereas fibroblasts or neurons show only a low level of antigen.Synonyms: H-2 class I histocompatibility antigen, H-2K(K), H2-K, H2-K1, K-K alpha chain