TCR gamma/delta antibody

Catalog No. ABIN371342

The Mouse Monoclonal anti-TCR gamma/delta antibody is suitable to detect TCR gamma/delta in samples from Chicken. It has been validated for FACS, IP, IHC (fro) and Func.

Quick Overview for TCR gamma/delta antibody (ABIN371342)

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCR gamma/delta antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: TCR1

Product Details anti-TCR gamma/delta Antibody

Characteristics: Synonyms: TCRG, TCRD, T-Cell Receptor gamma, T-Cell Receptor delta, T-Cell Receptor gamma delta,T-cell surface glycoprotein CD3 gamma/delta, T-cell receptor T3 gamma/delta

Purification: Purified

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. (Ref.1-3)Immunohistochemistry (Acetone-Fixed, Frozen Tissue Sections). (Ref.5)Immunoprecipitation. (Ref.1-3)In ovo depletion of gamma delta T cells. (Ref.6,7)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TCR gamma/delta

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Alternative Name: T Cell Receptor / TCR gamma/delta

Background: T cell receptors (TCR) recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. Unlike human and mouse, in which gamma/delta cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of gamma/delta T cells. The frequency of TCR gamma/delta cells is usually 20-25 % of the total blood T cells, but may reach approximately 50 % in chickens of 6 months of age. The majority of TCRgamma/delta+ cells in the thymus and blood are CD4-CD8-, although a small subset of them may express CD8 or CD4 coreceptors. However, when the TCRgamma/delta+ cells migrate into the spleen and intestine, most of them begin to express CD8. Although the biological function of gamma/delta T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ gamma/delta T cells may also be involved in down-regulation of the immune response. They cannot, however, induce a graft-vs-host (GVH) reactions.Synonyms: T-Cell Receptor delta, T-Cell Receptor gamma, T-Cell Receptor gamma delta, TCR delta, TCR gamma, TCRD, TCRG