Mouse anti-Human IgM Antibody (APC)

Catalog No. ABIN371452

Quick Overview for Mouse anti-Human IgM Antibody (APC) (ABIN371452)

Target: IgM

Clonality: Monoclonal

Conjugate: APC

Application: Immunofluorescence (IF)

Clone: SA-DA4

Isotype: IgG1

Product Details

Reactivity: Human

Host: Mouse

Application Details

Application Notes: Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 0.2 μg/10^6cells.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Comment:

Excitation/Emission wavelength: 650 nm/660 nm

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and a stabilizing agent.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Should it contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details

Target: IgM

Target Type: Antibody

Background: IgM normally constitutes about 10 % of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. Due to it's high valency IgM is more efficient than other isotypes is binding antigens with repeating epitopes (virus particles and red blood cells) and is more efficient than IgG in activiating the complement pathway. The gene for the mu constant region contains four domains separated by short intervening sequences. IgM measurement yields information about the body's immediate resistance and response to infection as well as information related to specific diseases. Decreased levels are associated with immune deficiency states, hereditary deficiencies, and myeloma. Increased levels can be associated with Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease.Synonyms: Human Immunoglobulin M