Mouse anti-Human IgG (Chain gamma) Antibody

Catalog No. ABIN371478

Quick Overview for Mouse anti-Human IgG (Chain gamma) Antibody (ABIN371478)

Target: IgG

Clonality: Monoclonal

Application: Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: JDC-10

Isotype: IgG1

Product Details

Binding Specificity: Chain gamma

Reactivity: Human

Host: Mouse

Purification: Purified

Application Details

Application Notes: Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 μg/10^6 cells. ELISA. Western blotting. Dot- and Slot-Immunoblotting. Immunohistochemistry on Frozen Sections. Immunocytochemistry.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgG

Target Type: Antibody

Background: IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75 % of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66 %), IgG2 (23 %), IgG3 (7 %) and IgG4 (4 %).Synonyms: Human Immunoglobulin G