Mouse anti-Human IgG Antibody (FITC)

Catalog No. ABIN371484

Quick Overview for Mouse anti-Human IgG Antibody (FITC) (ABIN371484)

Target: IgG

Clonality: Monoclonal

Conjugate: FITC

Application: Immunofluorescence (IF)

Clone: H2

Isotype: IgG2b

Product Details

Reactivity: Human

Host: Mouse

Application Details

Application Notes: Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 1 μg/10^6 cells.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.
This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -28 °C for longer.

Target Details

Target: IgG

Target Type: Antibody

Background: IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75 % of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66 %), IgG2 (23 %), IgG3 (7 %) and IgG4 (4 %).Synonyms: Human Immunoglobulin G