TLX1 antibody (AA 300-330)

Catalog No. ABIN372448

This Rabbit Polyclonal antibody specifically detects TLX1 in WB and IHC (p). It exhibits reactivity toward Mouse, Human and Rat.

Quick Overview for TLX1 antibody (AA 300-330) (ABIN372448)

Target: TLX1 (T-Cell Leukemia Homeobox 1 (TLX1))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TLX1 Antibody

Binding Specificity: AA 300-330

Specificity: This antibody recognizes Homeobox Protein Hox-11 (TLX1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Protein G Chromatography.

Immunogen: Synthetic peptide corresponding to a portion of amino acids 300-330 of human HOX11.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Sections: 5 μg/mL. Western blot: 1-3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.2 % Gelatin as stabilizer and 0.05 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for TLX1

Target: TLX1 (T-Cell Leukemia Homeobox 1 (TLX1))

Alternative Name: TLX1

Background: HOX11, also called homeobox11 or Tcl-3 is located at the 10q24 T-Cell translocation breakpoint region. It encodes a homeobox-domain containing protein, HOX11, containing a glycine and proline-rich amino terminus. HOX11 is required for maintenance of the developing spleen and cell survival. The HOX11protein binds to a specific DNA sequence and localizes to the cell nucleus. It transactivates transcription of a reporter gene linked to a cis-regulatory element, acting as a positive transcription activator. The HOX11 gene is highly expressed in T-ALLs as a result of a t (7:10) (q35,q24) or t(10:14)(q24,qll) translocation. The homeobox gene is deregulated upon translocation into the T-cell receptor locus and activates a specific subset of tumor-associated target genes. HOX 11 gene translocation has been attributed to T-cell leukemia and lymphoid neoplasias.Synonyms: HOX11, Hox-11, T-cell leukemia homeobox protein 1, T-cell leukemia/lymphoma protein 3, TCL-3, TCL3, TLX-1

Gene ID: 3195

NCBI Accession: NP_005512

UniProt: P31314