14-3-3 Pan (pSer58) antibody

Antigen: 14-3-3 Pan

Epitope: pSer58

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN372585

Additional Information

Characteristics: Synonyms: YWAH

Alternative name: 14-3-3 protein pan

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 ofrat 14-3-3 protein.

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Primate, Rat (Rattus), Sheep (Ovine), Xenopus laevis

Isotype: IgG  (Matching secondary antibodies)

Description: 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple rolesin cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed inthe brain and have been detected in the cerebrospinal fluid of patients with differentneurological disorders (Berg et al. , 2003). 14-3-3 proteins bind protein ligands that aretypically phosphorylated on serine or threonine residues and regulate the functions ofthese binding partners by a number of different mechanisms (Silhan et al. , 2004,Dougherty and Morrison, 2004). The14-3-3 proteins affect a diverse array of cellularprocesses including the cell cycle and transcription, signal transduction and intracellulartrafficking. These functions of 14-3-3 proteins are facilitated by, if not dependent on, itsdimeric structure. Recent work has demonstrated that the dimeric status of the 14-3-3protein is regulated by site-specific serine phosphorylation (Woodcock et al. , 2003).

Specificity: Specific for the ~29k 14-3-3 protein phosphorylated at Ser58. Immunolabeling is blocked by the phosphopeptide used as antigen but not by thecorresponding dephosphopeptide. Species Reactivity: Tested: Human and Rat. Expected from sequence similarity: Bovine, Canine, Chicken, Mouse, Sheep, Xenopus,Zebrafish and non-Human Primates.

Application Details

Application Notes: Western Blot: 1/1000. Immunohistochemistry: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Aff - Purified

Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% Glycerol.

Storage: Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only