Periostin, Osteoblast Specific Factor (POSTN) (C-Term) antibody

Antigen: Periostin, Osteoblast Specific Factor (POSTN)

Synonyms: PN, OSF2, OSF-2, PDLPOSTN, MGC119510, MGC119511, periostin, RP11-412K4.1, PLF, Osf2, peri, AI747096, Periostin, MGC128214, pn, fa99h07, MGC153873, wu:fa99h07, wu:fc70f09, zgc:153873, LOC100008945, osf-2, pdlpostn

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Chicken

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN372700

Additional Information

Characteristics: Synonyms: POSTN, OSF2, OSF-2, PN, Osteoblast-specific factor 2

Alternative name: Periostin

Swiss-Prot: Q15063

Immunogen: Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminalregion of mouse Periostin which is comprised of six small alternatively-spliced exons.

Cross-Reactivity: Chicken, Human, Mammalian, Mouse (Murine), Rat (Rattus)

Isotype: IgG  (Matching secondary antibodies)

Description: Periostin is a matricellular protein, i. e. an extracellular matrix protein that interacts bothwith other ECM proteins and with cell-surface receptors. Like many other matricellularproteins, the function of periostin is important both in embryonic development and in theremodeling of adult tissues in response to pathological insults. Periostin was originallyisolated as an osteoblast-specific marker that functions as a cell adhesion molecule forpreosteoblasts and is thought to be involved in osteoblast recruitment, attachment andspreading (Kruzynska-Frejtag A. et al. , 2004). Periostin has recently been shown topromote collagen fibrogenesis, inhibit differentiation of progenitor cells intocardiomyocytes and to be essential in maintaining the biomechanical properties of theadult myocardium (Norris et al. , 2008)

Specificity: This antibody recognizes the ~93 kDa Periostin doublet in mouse lung extract. Thisantibody does not recognize the single heart form of Periostin or the lowest molecularweight form of lung periostin. Consistent with the fact that this antibody is made againstan alternatively spliced region of periostin, this antibody recognizes a subset of the formsof Periostin recognized on Western blots by the Pan Periostin antibody (Cat#ABIN372701)and also shows a distinctive staining pattern by Immunohistochemistry (data not shown). Species Reactivity: Tested: Human, Mouse and Chicken. Expected from sequence similarity: Mammalia and Avian.

Synonyms: PN, OSF2, OSF-2, PDLPOSTN, MGC119510, MGC119511, periostin, RP11-412K4.1, PLF, Osf2, peri, AI747096, Periostin, MGC128214, pn, fa99h07, MGC153873, wu:fa99h07, wu:fc70f09, zgc:153873, LOC100008945, osf-2, pdlpostn

Application Details

Application Notes: Western blot: 1/1000. Immunohistochemistry: 1/100. The antibody works well for Immunohistochemistry on paraformaldehyde-fixed sectionswith a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90ºC in 10 mMsodium citrate (pH 6.0)/ 0.1% Tween-20). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Azide Free

Buffer: PBS without preservatives.

Storage: Store the antibody undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only