PNPLA2 antibody (Internal Region)

Catalog No. ABIN372772

This Goat Polyclonal antibody specifically detects PNPLA2 in IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PNPLA2 antibody (Internal Region) (ABIN372772)

Target: PNPLA2 (Patatin-Like phospholipase Domain Containing 2 (PNPLA2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PNPLA2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PNPLA2 Antibody

Binding Specificity: Internal Region

Specificity: Recognizes Patatin-Like Phospholipase Domain Containing 2 (PNPLA2).

Cross-Reactivity (Details): Species reactivity (expected):Human and Rat.
Species reactivity (tested):Mouse.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide (C-NIIEVSKEARKR) from an internal region of Mouse PNPLA2.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/32000. Immunohistochemistry on Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PNPLA2

Target: PNPLA2 (Patatin-Like phospholipase Domain Containing 2 (PNPLA2))

Alternative Name: PNPLA2 / ATGL

Background: Adipose triglyceride lipase (ATGL) may function as a lipase and play a role in the adaptive response to a low energy state, such as fasting, by providing fatty acids to other tissues for oxidation. In addition, decreased expression of desnutrin in obesity models suggests its possible contribution to the pathophysiology of obesity. ATGL catalyzes the initial step in triglyceride hydrolysis in mammalian adipose tissue. ATGL contains a patatin domain common to plant acyl hydrolases. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammalsSynonyms: Adipose triglyceride lipase, Calcium-independent phospholipase A2, Desnutrin, FP17548, IPLA2-zeta, Patatin-like phospholipase domain-containing protein 2, Pigment epithelium-derived factor, TTS2.2, Transport-secretion protein 2

Gene ID: 57104

NCBI Accession: NP_065109

UniProt: Q96AD5