PC antibody (Internal Region)

Catalog No. ABIN372799

This Goat Polyclonal antibody specifically detects PC in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow and Dog.

Quick Overview for PC antibody (Internal Region) (ABIN372799)

Target: PC (Pyruvate Carboxylase (PC))

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PC Antibody

Binding Specificity: Internal Region

Specificity: Recognizes Pyruvate Carboxylase (PC).

Cross-Reactivity (Details): Species reactivity (expected):Bovine, Canine (Dog), Mouse, Porcine and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide (C-KFKEVKKAYVEANQ) from an Internal region of Human PC.

Isotype: IgG

Application Details

Application Notes: ELISA: 1/16000. Western blot: 0.03-0.1 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PC

Target: PC (Pyruvate Carboxylase (PC))

Alternative Name: Pyruvic Carboxylase (PC)

Background: Pyruvate Carboxylase (PC, PCB) requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. PCB is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.Synonyms: PCB, Pyruvate carboxylase mitochondrial

Gene ID: 5091

NCBI Accession: NP_000911

UniProt: P11498